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Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput sequencing analyses

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

《环境科学与工程前沿（英文）》 2016年 第10卷 第3期   页码 539-547 doi: 10.1007/s11783-016-0830-5

摘要： This study employed 454-pyrosequencing to investigate microbial and pathogenic communities in two wastewater reclamation and distribution systems. A total of 11972 effective 16S rRNA sequences were acquired from these two reclamation systems, and then designated to relevant taxonomic ranks by using RDP classifier. The Chao index and Shannon diversity index showed that the diversities of microbial communities decreased along wastewater reclamation processes. was the most dominant phylum in reclaimed water after disinfection, which accounted for 83% and 88% in two systems, respectively. Human opportunistic pathogens, including , , and , were selected and enriched by disinfection processes. The total chlorine and nutrients (TOC, NH -N and NO -N) significantly affected the microbial and pathogenic communities during reclaimed water storage and distribution processes. Our results indicated that the disinfectant-resistant pathogens should be controlled in reclaimed water, since the increases in relative abundances of pathogenic bacteria after disinfection implicate the potential public health associated with reclaimed water.

关键词： wastewater reclamation systems     microbial community     pathogenic community     454-pyrosequencing    

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Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1504-5

摘要：

• Diversity and detection methods of pathogenic microorganisms in sludge.

关键词： Sludge treatment     Pathogenic microorganisms     Inactivation mechanisms     Exposure risks     Land application    

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Assessment of vaccination strategies against highly pathogenic avian influenza in China

Honglei SUN,Jinhua LIU

《农业科学与工程前沿（英文）》 2014年 第1卷 第4期   页码 277-281 doi: 10.15302/J-FASE-2014036

摘要： Vaccination for highly pathogenic avian influenza (HPAI) has been implemented in China for a decade, however, the virus is still present in poultry. A series of recombinant vaccines, Re-1 to Re-7, have been developed and used, and Re-8 will also be used in clinical settings to prevent the prevailing flu strains. The question remains, when can China eradicate the disease? Here, we review the epidemiology of H5 HPAI along with the development, usage and problems of vaccines. Further suggestions for controlling the disease in China are provided.

关键词： highly pathogenic avian influenza     vaccine     vaccination     control    

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IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE -2020343

摘要：

The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of Eucalyptus tereticornis, Xanthium sibiricum, Artemisia argyi, Tupistra chinensis and Pyrola calliantha were evaluated against the mycelial growth of the plant pathogenic fungi Aspergillus niger, Botrytis cinerea, Penicillium digitatum, P. expansum, P. italicumand Rhizopus stolonifer. All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol (v/v), 95% ethanol (v/v), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from T. chinensis rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. T. chinensis may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

 

关键词： Botrytis cinerea / food preservation / plant diseases / plant pathogenic fungi / Tupistra chinensis    

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Research progress in pathogenic genes of hereditary non-syndromic mid-frequency deafness

null

《医学前沿（英文）》 2016年 第10卷 第2期   页码 137-142 doi: 10.1007/s11684-016-0449-8

摘要：

Hearing impairment is considered as the most prevalent impairment worldwide. Almost 600 million people in the world suffer from mild or moderate hearing impairment, an estimated 10% of the human population. Genetic factors play an important role in the pathogenesis of this disorder. Hereditary hearing loss is divided into syndromic hearing loss (associated with other anomalies) and non-syndromic hearing loss (not associated with other anomalies). Approximately 80% of genetic deafness is non-syndromic. On the basis of the frequency of hearing loss, hereditary non-syndromic hearing loss can be divided into high-, mid-, low-, and total-frequency hearing loss. An audiometric finding of mid-frequency sensorineural hearing loss, or a “bowl-shaped” audiogram, is uncommon. Up to now, merely 7 loci have been linked to mid-frequency hearing loss. Only four genetic mid-frequency deafness genes, namely, DFNA10 (EYA4), DFNA8/12 (TECTA), DFNA13 (COL11A2), DFNA44 (CCDC50), have been reported to date. This review summarizes the research progress of the four genes to draw attention to mid-frequency deafness genes.

关键词： hereditary non-syndromic hearing loss     mid-frequency hearing loss     deafness genes    

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The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

《环境科学与工程前沿（英文）》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1356-4

摘要：

•Bacterial concentrations from eight stages were 104–105copies/m3.

关键词： Size distribution     Airborne bacteria     Biological diversity     Human pathogenic bacteria     Composting plants    

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ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

《农业科学与工程前沿（英文）》 2021年 第8卷 第4期   页码 635-644 doi: 10.15302/J-FASE-2020343

摘要： The antifungal effectiveness of extracts of five medicinal plant species was determined. The inhibitory activity of extracts of , , , and were evaluated against the mycelial growth of the plant pathogenic fungi , , and . All plant extracts were prepared at 60°C using solvents (either water, 50% ethanol ( ), 95% ethanol ( ), ethyl acetate or petroleum ether). Fungicidal effects of all plants tested were confirmed. Different extracts from the same plant species gave different degrees of inhibition. All aqueous extracts had weak or no activity on all fungi tested. Ethyl acetate and 95% ethanol extracts from rhizomes gave greater inhibition and a broader spectrum inhibition than the other extracts. may have potential as a new natural fungicide and may be used for the preservation of agricultural and forestry products such as fruits and vegetables.

关键词： Botrytis cinerea     food preservation     plant diseases     plant pathogenic fungi     Tupistra chinensis    

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R158Q and G212S, novel pathogenic compound heterozygous variants in of Gitelman syndrome

《医学前沿（英文）》 2022年 第16卷 第6期   页码 932-945 doi: 10.1007/s11684-022-0963-9

摘要： The dysfunction of Na+-Cl− cotransporter (NCC) caused by mutations in solute carrier family12, member 3 gene (SLC12A3) primarily causes Gitelman syndrome (GS). In identifying the pathogenicity of R158Q and G212S variants of SLC12A3, we evaluated the pathogenicity by bioinformatic, expression, and localization analysis of two variants from a patient in our cohort. The prediction of mutant protein showed that p.R158Q and p.G212S could alter protein’s three-dimensional structure. Western blot showed a decrease of mutant Ncc. Immunofluorescence of the two mutations revealed a diffuse positive staining below the plasma membrane. Meanwhile, we conducted a compound heterozygous model—Ncc R156Q/G210S mice corresponding to human NCC R158Q/G212S. NccR156Q/G210S mice clearly exhibited typical GS features, including hypokalemia, hypomagnesemia, and increased fractional excretion of K+ and Mg2+ with a normal blood pressure level, which made NccR156Q/G210S mice an optimal mouse model for further study of GS. A dramatic decrease and abnormal localization of the mutant Ncc in distal convoluted tubules contributed to the phenotype. The hydrochlorothiazide test showed a loss of function of mutant Ncc in NccR156Q/G210S mice. These findings indicated that R158Q and G212S variants of SLC12A3 were pathogenic variants of GS.

关键词： Gitelman syndrome     mouse model     compound heterozygous     hypokalemia     Slc12a3    

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Fungal diversity and its mechanism of community shaping in the milieu of sanitary landfill

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1370-6

摘要：

• Ascomycota was the predominant phylum in sanitary landfill fungal communities.

关键词： Sanitary landfill     Fungal community     Diversity     Saprotroph     Physical habitat     Environmental factor    

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Avian influenza A: an avian influenza virus from low pathogenic to highly pathogenic

William J. Liu, Haixia Xiao, Lianpan Dai, Di Liu, Jianjun Chen, Xiaopeng Qi, Yuhai Bi, Yi Shi, George F. Gao, Yingxia Liu

《医学前沿（英文）》 2021年 第15卷 第4期   页码 507-527 doi: 10.1007/s11684-020-0814-5

摘要： The avian influenza A (H7N9) virus is a zoonotic virus that is closely associated with live poultry markets. It has caused infections in humans in China since 2013. Five waves of the H7N9 influenza epidemic occurred in China between March 2013 and September 2017. H7N9 with low-pathogenicity dominated in the first four waves, whereas highly pathogenic H7N9 influenza emerged in poultry and spread to humans during the fifth wave, causing wide concern. Specialists and officials from China and other countries responded quickly, controlled the epidemic well thus far, and characterized the virus by using new technologies and surveillance tools that were made possible by their preparedness efforts. Here, we review the characteristics of the H7N9 viruses that were identified while controlling the spread of the disease. It was summarized and discussed from the perspectives of molecular epidemiology, clinical features, virulence and pathogenesis, receptor binding, T-cell responses, monoclonal antibody development, vaccine development, and disease burden. These data provide tools for minimizing the future threat of H7N9 and other emerging and re-emerging viruses, such as SARS-CoV-2.

关键词： H7N9     HPAIV     epidemiology     clinical features     pathogenesis     hemagglutinin     immunity     vaccine    

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of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community

《环境科学与工程前沿（英文）》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1576-x

摘要：

● Presented coupled system enhanced biodegradation of antibiotic chloramphenicol.

关键词： Electrical stimulation     Biodegradation     Microbial community     Chloramphenicol    

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Distinct community assembly processes underlie significant spatiotemporal dynamics of abundant and rare

《环境科学与工程前沿（英文）》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1513-4

摘要：

• Season and landform influenced spatiotemporal patterns of abundant and rare taxa.

关键词： Rare taxa     Biogeography     Community assembly     Bacterioplankton     The Yangtze River    

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Attributes, challenges and future directions of community resilience

Bo MENG, Nan LI, Dongping FANG

《工程管理前沿（英文）》 2018年 第5卷 第3期   页码 307-323 doi: 10.15302/J-FEM-2018030

摘要：

With the continuous development of human society, the damage to the natural environment is becoming increasingly large, causing crisis events to occur frequently. In recent years, the study of community resilience is becoming popular among scholars because of its perspective on disaster prevention and mitigation. In this study, we apply database retrieval to untangle community resilience-related papers from multiple directions. We analyze the connotation, attribute, and composition of community, resilience, and community resilience comprehensively by summarizing important issues and research progress in community resilience. Challenges and shortcomings faced by community resilience development are also determined. Moreover, we put forward the research directions that future research can focus on. Through literature review, current research on community resilience focuses on the following aspects: “infrastructure construction,” “crowd in the community,” “economic resilience,” “social capital,” and “measurement of community resilience.” Such research is not yet systematic and relatively decentralized. The attention to collective resilience in the community is relatively weak, which makes achieving the goal of “people-oriented and systematic control” difficult. Therefore, this study states that the development of future community resilience should stand from the perspective of “system of system” and build on knowledge and tools of various relevant domains. Therefore, public needs and participation are highlighted as breakthrough points. The research should integrate infrastructure and economic resilience, social resource allocation, network connection, and other aspects to build a holistic and functional resilient community.

关键词： community resilience     literature analysis     resilience attribute     social resource     future direction    

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The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yadong YANG, Xiaomin FENG, Yuegao HU, Zhaohai ZENG

《农业科学与工程前沿（英文）》 2019年 第6卷 第2期   页码 162-171 doi: 10.15302/J-FASE-2018212

摘要：

In this study, the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil in three cropping systems and at two oat growth stages were investigated using real-time PCR and Illumina MiSeq sequencing. The gene abundance in oat-soybean intercropping (OSO) and oat-mungbean intercropping (OMO) was significantly greater than that in sole oat (O), but the gene abundance significantly decreased at the later stage in all the treatments. Alpha diversity indices in OSO and OMO were higher at the heading stage, but lower at the maturity stage than that in O. and were the dominant genera identified in all samples, with an average proportion of 35.8% and 12.4%, respectively. The proportion of dominant genera showed significant differences and varied with cropping system and growth stage. Principal component analysis showed that growth stage had a stronger effect than intercropping on the diazotrophic community structure. However, Mantel test and redundancy analysis showed there was no environmental factor significantly correlated to the diazotrophic community structure. Our results demonstrate that intercropping had a weaker effect than growth stage on the abundance, diversity and structure of the diazotrophic community in oat rhizosphere soil.

关键词： community composition     Illumina MiSeq sequencing     nifH gene     oat-legume intercropping     rhizosphere soil    

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Insights into the changes of amino acids, microbial community, and enzymatic activities related with

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1635-y

摘要：

● The highest seed germination index was achieved at 0.3 g/g total solids of food waste.

关键词： Composting     Food waste     Amino acids     Microbial community     Enzymatic activity     Nutrient quality    

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标题 作者 时间 类型 操作

Changes of microbial composition during wastewater reclamation and distribution systems revealed by high-throughput sequencing analyses

Yiwen LIN,Dan LI,Siyu ZENG,Miao HE

期刊论文

Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

期刊论文

Assessment of vaccination strategies against highly pathogenic avian influenza in China

Honglei SUN,Jinhua LIU

期刊论文

IN VITRO ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

期刊论文

Research progress in pathogenic genes of hereditary non-syndromic mid-frequency deafness

null

期刊论文

The size distribution of airborne bacteria and human pathogenic bacteria in a commercial composting plant

期刊论文

ACTIVITY OF EXTRACTS OF FIVE MEDICINAL PLANT SPECIES ON PLANT PATHOGENIC FUNGI

Shufeng YAO, Jiali XU, Haibo ZHANG, Hong GAO, Shibin SHANG, Dan WANG

期刊论文

R158Q and G212S, novel pathogenic compound heterozygous variants in of Gitelman syndrome

期刊论文

Fungal diversity and its mechanism of community shaping in the milieu of sanitary landfill

期刊论文

Avian influenza A: an avian influenza virus from low pathogenic to highly pathogenic

William J. Liu, Haixia Xiao, Lianpan Dai, Di Liu, Jianjun Chen, Xiaopeng Qi, Yuhai Bi, Yi Shi, George F. Gao, Yingxia Liu

期刊论文

of different voltage application modes on biodegradation of chloramphenicol and shift of microbial community

期刊论文

Distinct community assembly processes underlie significant spatiotemporal dynamics of abundant and rare

期刊论文

Attributes, challenges and future directions of community resilience

Bo MENG, Nan LI, Dongping FANG

期刊论文

The diazotrophic community in oat rhizosphere: effects of legume intercropping and crop growth stage

Yadong YANG, Xiaomin FENG, Yuegao HU, Zhaohai ZENG

期刊论文

Insights into the changes of amino acids, microbial community, and enzymatic activities related with

期刊论文